ABSTRACT
Delayed-type hypersensitive (DTH) response was evaluated in C3H/HeJ mice intestinally infected with Entamoeba histolytica. Infected and non-infected control mice were challenged with amoebic antigen on day 5, 10, 15, 20, 25, 30, 40, 50, and 60 post-infection. Maximum footpad swelling was observed after 24 hours of the challenge. The E. histolytica-infected mice exhibited a DTH response on day 5, 15, 20, 25, 40 and 60 post-infection. However, on day 10, 30, and 50, such response was similar to that of the non-infected control mice. The mice developed an evident DTH response late in the course of infection (25 days post-infection). The infected mice did not show any alteration to their DTH response against heterologous unrelated antigen (sheep red blood cells), suggesting that cellular anergy was antigen-specific.
Subject(s)
Animals , Disease Models, Animal , Dysentery, Amebic/immunology , Entamoeba histolytica/isolation & purification , Hypersensitivity, Delayed , Immunity, Cellular/immunology , Mice , Mice, Inbred C3H , Time FactorsABSTRACT
The reactivity of sera from experimentally infected animal was studied from 5-60 days postinoculation to determine which of the E. histolytica antigens are recognized frequently to infection. Crude extract of E. histolytica trophozoites was used and sera were examined by immunoelectrotransference assay. It was observed that sera recognized polypeptide with 70 kDa molecular mass after 15 days postinoculation onward and later 14 to 24 polypeptide with molecular weight of 110-22 kDa were recognized. All the amebic antigens (polypeptides) could be recognized by sera till 60 days postinoculated animals. Significance of expression of different amebic polypeptides in terms of pathogenesis needs further investigations.
Subject(s)
Animals , Antigen-Antibody Reactions , Antigens, Protozoan/chemistry , Entamoeba histolytica/immunology , Entamoebiasis/immunology , Enteritis/immunology , Immunoglobulin G/immunology , Mice , Mice, Inbred C3H , Molecular WeightABSTRACT
En el presente reporte informamos sobre la obtencion de 15 hibridomas que secretan anticuerpos contra antigenos de Entamoeba. De estos hibridos, 5 han sido subclonados para obtener un total de 21 subclonas, la cuales secretan anticuerpo monoclonal de la subclase IgG, de tipo k. De las clonas aisladas se seleccionaron arbitrariamente 6 que reaccionaron con trofozoitos de Entamoeba histolytica de las cepas HM1:IMSS y HK9:NIH. Sin embargo, solo una de ellas reacciono con una cepa de E. moshkovskii